![]() ![]() ![]() The sizes of the two bubbles remain similar, and the bilayer shape is flat without discernible curvature ( Figure 1A). Water bubbles with azolectin as lipids are blown into an oil phase (hexadecane) by manipulating the pneumatic injectors until the desired size, such as 100 μm in diameter, is reached, followed by fine-tuning the injectors to maintain the size (see methods in the Supporting Information for details). The pressure in the bubble relative to the atmosphere must be defined appropriately, and the capillary action and gravity are taken into account (described in detail in the Materials and Methods section). The fine pressure gauge continuously monitors the intrabubble pressure, p, for each bubble. The core of the present method lies in precise measurement of the low intrabubble pressure in the range of 100 Pa ( Figure 1). ![]()
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